Relationship between expression of E26 transformation-specific-1(Ets-1)and tumor angiogenesis,cancer cell prolif eration,invasion and metastasis in cervical cancer
AIM: To investigate the expressions of E26 trans-formation-specific-1 (Ets-1) proto-oncogene protein, microvessel density (MVD) and cell proliferation nuclear antigen-Ki67 in cervical cancer tissue, and to explore the role of Ets-1 in the development, growth, invasion and metastasis of cervical cancer. METHODS: Immunohistochemical staining-SP method was used to detect the expressions of Ets-1, MVD (labeled by CD34) and cell proliferation nuclear antigen -Ki67 in 67 cases of cervical cancer. The relationships between Ets-1 and the clinicopathologi-cal variables, MVD and Ki67 were analyzed by Student's t-test and Chi-square analysis. RESULTS: Ets-1 was highly expressed in tumor tissues (42/67, 62. 7% ) and Ets-1 was not found in normal tissues, and the difference between the groups was significant (χ2 =14.94, P=0.001). MVD was 14.91 ±2.55 in normal tissues and 37. 57 ± 6. 77 in tumor tissues, with significant difference between the groups (t = 10. 920, P =0. 000). The MVD of Ets-1 -positive tumors was higher than that of Ets-1-negative tumors, with significant difference (P = 0.000). Positive rate of Ki67 in tumors was 95.5% (64/67) , significantly higher than that in normal tissue (χ2 =44.292, P=0.000). There was a significant correlation between the expressions of Ets-1 and Ki67 (R =0.422, P=0.002). There were significant correlations between the expressions of Ets-1 and increased clinical stage ( R = 0.355, P=0.002) and histopathological grading (R = 0.421, P =0.006). The expressions of Ets-1 was significantly higher in patients with lymph node metastasis and deep muscle invasion of the tumors compared with the patients with no lymph node metastasis and superficial muscle invasion( P <0.01). CONCLUSION: Ets-1 may accelerate the development and metastasis of cervical canc- er through promoting tumor angiogenesis and cell proliferating. Ets-1 may be a valuable marker to evaluate biological behavior of cervical cancer and to predict the outcome for patients with cervical cancer.
NETL
NSTL
万方数据
维普
