红花的药效差示血清色谱法研究
Study on differential efficacy of ser um chromatography of Carthamus tinctoriu L
杨静 1杨志福 1陈敏纯 1乔逸 2田云 1贾艳艳 1文爱东1
作者信息
- 1. 第四军医大学西京医院药剂科,陕西,西安,710033
- 2. 陕西中医学院药学院,陕西,咸阳,712046
- 折叠
摘要
目的:首次采用以药效为导示的差示血清色谱法探索红花在大鼠体内的药效物质基础.方法:采用高效液相色谱(High-performance liquid chromtogrwhy,HPLC)指纹图谱的分析方法,比较红花提取物及服用红花提取物后最大药效时刻、最小药效时刻的含药血清色谱图,探索红花在血瘀大鼠体内发挥药效的物质基础.结果:血瘀大鼠灌胃给药后血清中产生药源性成分28个,其中加个成分为红花原形成分,其余8个为新产生的代谢产物.结论:红花体内直接作用物质很可能出自血清中的28个药源性成分,对其进行深入研究将有助于阐明红花的有效成分及作用机制.
Abstract
AIM: To investigate the effective constituents of Carthamus tinctoriu L in rats using differential efficacy of serum chromatography. METHODS: High-performance liquid chroma-togmphy(HPLC) fingerprint was employed to compare the peaks of Carthamus tinctoriu L extract, blank serum, drug-containing serum at maximum efficacy time and drug-containing serum at minimum efficacy time. The chemical componential foundation of Carthamus tinctoriu L efficacy in blood stasis rats was determined. RESULTS: Twenty-eight compounds were detected after intragas-tric administration of Carthamus tinctoriu L extract, 20 of which were the original compounds containing in Carthamus tinctoriu L extract and 8 were metabolites. CONCLUSION: The 28 compounds under study are the possible effective constituents and further study may add to our understanding of the pharmacology and active mechanism of Carthamus tinctoriu L.
关键词
红花/药效差示血清色谱/色谱法/高压液相/指纹图谱Key words
carthamus tinctorius/differential efficacy of serum chromatography/chromatography/high pressure liquid/fingerprint引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据