Effects of Saponins of Astragalus on differentiation of neural stem cells
AIM: To observe the effects of Saponins of Astragalus (SA) of various concentrations on the differentiation of neural stem cells (NSCs). METHODS: NSCs from the anterior subven-tricular zone of the embryonic mouse( E14. 5) were generated by mechanical isolation and serum-free culture. To study the effects of different concentrations (20,40,60 mg/L) of SA on the differentiation of the NSCs at different stages (3 and 7 d),we adopted immunofluorescence to examine the proportion of the neuron (β-Tubulin),astrocyte(GFAP) and oligodendrocyte (CC-1) by adding different concentrationsof SA. 40 mg/L Ginkgolide B (GKB) was employed as positive control of SA and normal medium was employed as negative control. RESULTS: The proportion of β-Tubulin positive neurons significantly increasedafter 3 and 7 d culture with SA of 40 and 60 mg/L and the GKB and no significant difference was observed between 40 and 60 mg/L of SA. The proportion of GFAP positive astrocytes increased significantly with the adding of GKB,while SA had no obvious effects on the differentiation of the astrocytes. CONCLUSION: Saponins of Astragalus specifically promotes NSCs to differentiate into neurons,while GKB promotes the NSCs to differentiate into both neurons and astrocytes.
saponins of astragalusginkgolide Bneural stem cellsdifferentiation
NETL
NSTL
万方数据
维普
