Astragalus membranaceu reduces ischemia-reperfusion injury of rat liver graft by inhibiting NF-κB
AIM: To investigate the protective role of astragalus membranaceu ( AM) in ischemic reperfusion injury of rat liver graft and its mechanism. METHODS: One hundred and twenty-eight male Sprague Dawley (SD) rats were randomly divided equally into AM group and NS group and were used as donors and recipients of orthotopic liver transplantation. One hour,2 h and 4 h following liver transplantation,samples of blood and hepatic tissues were taken for assay. Amounts of TNF-α (tumor necrosis factor-α) and ALT ( alanine aminotransferase) values in serum were detected. The activity of NF-κB (nuclear factor kappa B) was determined by immunohistochemical staining and ELISA,and the expression of ICAM-1 ( intercellular adhesion molecule-1 ) protein level was determined by Western Blotting. Three days following the transplantation,apoptosis indexes were observed by TUNEL. RESULTS: In AM group ALT values were (9. 4 ± 0.4),(10. 8 ± 0. 5) and (14. 3 ± 1. 1) μkat/L respectively,which were lower than(12.4 ±0. 6),(15.9 ±0.8) and(18.7± 1.1) μkat/L respectively in NS group(P <0. 05). TNF-α concentrations were(757± 54),(977 ±60) and(318 ± 53) ng/L,which were also lower than (1258 ± 132),(1831 ± 165 ) and (499 ±66) ng/L respectively in NS group(P <0.05). Expression of ICAM-1 and the activity of NF-κB in AM group were also lower than those in NS group ( P < 0. 05 ). The apoptosis indexes in the AM group were lower than those in NS group ( P < 0. 05 ). Histological examination revealed that the injuries of hepatic tissues in NS group were more severity than those in AM group. CONCLUSION: Astragalus membranaceu protects hepatic I/R injury after liver transplantation,improves liver graft function and reduces the release of inflammatory mediators.
NETL
NSTL
万方数据
维普
