Shear stress induces differentiation of bone marrow mesenchymal stem cells into endothelial cells
AIM: To examine the effect of shear stress on the differentiation of bone marrow ( MSCs ). METHODS: Parallel plate flow chamber system of shear stress was used. MSCs were subjected to different levels of shear stress of 3 ×10 -5,8 ×10-5 and 15 × 10-5 N/cm2 respectively for 24 h. Cell shape and orientation were observed at 0 h and 24 h during shear stress. Indirect IF staining was employed to identify the endothelial cell-specific marker Von Willebrand factor(vWF). A functional marker of mature endothelial cells was examined by the uptake of Dil tabled acetylated low-density lipoproteins ( Dil-Ac-LDL). RESULTS: After subjected to shear stress for 24 h,MSCs tended to be rounder and polygon-like in shape,and aligned parallel to the flow. MSCs subjected to shear stress of 3 × 10-5 and 8 × 10-5 N/cm2 were positive for both vWF staining and the uptaking of Dil-Ac-LDL,indicating respectively the endothelial phenotype and the possession of mature endothelial function. Cells under shear stress of 15 × 10 -5 N/cm2 were negative for both vWF staining and the uptaking of Dil-Ac-LDL,indicating the lack of differentiation. CONCLUSION:Shear stress induces differentiation of bone marrow mesenchymal stem cells into endothelial cells in a stress level dependent way.
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万方数据
维普
