Expression of β-defensin in liver tissue and alteration of serum inflammatory factors in diabetic rats
AIM: To investigate the expression of β-defensin in liver tissues and alteration of serum inflammatory factors of diabetic rate so as to explore the possible interactive effect. METHODS: SD rats were divided into 4 groups: control group,experimental group,4 wk diabetic rats group,and 12 wk diabetic rats group. Inflammatory factors of blood serum TNF-α,IL-1β,IL-10 and p-defensin were examined by ELISA method,Western Blot and real tune polymerase chain reaction ( RT-PCR). RESULTS: During the whole course of diabetes,there was persistent activation of TNF-α and IL-1 β and ebb of anti-inflammatory factor IL-10. Expression of β-defensins1 ( rBD-1 ) was slight and expression of β-defensins2 ( rBD-2) decreased,which suggested that high glucose or glycosylation reaction probably interfered the constitution expression of rBD-2 and reduced the induction expression. CONCLUSION:There exists chronic inflammatory effect significantly in the diabetic liver. As a key role,the impairment of rBD-2 is probably induced by the high glucose and subsequent glycosylation reaction.
NETL
NSTL
万方数据
维普
