糖尿病大鼠肝脏β-defensins表达及血清炎症因子的变化
Expression of β-defensin in liver tissue and alteration of serum inflammatory factors in diabetic rats
蒙广星 1袁强 1王毅军 1孟化 2白飞虎3
作者信息
- 1. 天津市第三中心医院肝胆外科,天津,300170
- 2. 北京大学附属北京肿瘤研究所,北京,100142
- 3. 第四军医大学消化病研究所,陕西,西安,710033
- 折叠
摘要
目的:观察糖尿病大鼠病程中肝脏β-defensins的表达和血清炎症因子的变化,探讨在糖尿病状态时两者之间可能存在的影响.方法:实验大鼠分为普通饲料组、高糖饲料组、糖尿病4wk组和12wk组,采集血液检测血清炎症因子TNF-α,IL-1β及IL-10和血糖、胰岛素、三酰甘油等相关生化指标;收集肝脏标本,RT-PCR及Western Blot方法检测β-defensins的表达.结果:与对照组相比,糖尿病大鼠整个病程中都存在致炎因子TNF-α,IL-1β激活及抗炎因子IL-10的削弱.肝脏中表达β-defensins 1(rBD-1)和β-defensins 2(rBD-2).rBD-1呈少量表达,糖尿病病程中rBD-2表达呈减少趋势.高血糖或糖基化反应可能干扰rBD-2的组成性表达及炎症因子产生的诱导性表达.结论:糖尿病大鼠病程中存在慢性炎症,高血糖或糖基化反应干扰了炎症刺激重要的小分子抗菌肽β-defensins在肝脏表达的信号通路,使其表达下调.
Abstract
AIM: To investigate the expression of β-defensin in liver tissues and alteration of serum inflammatory factors of diabetic rate so as to explore the possible interactive effect. METHODS: SD rats were divided into 4 groups: control group,experimental group,4 wk diabetic rats group,and 12 wk diabetic rats group. Inflammatory factors of blood serum TNF-α,IL-1β,IL-10 and p-defensin were examined by ELISA method,Western Blot and real tune polymerase chain reaction ( RT-PCR). RESULTS: During the whole course of diabetes,there was persistent activation of TNF-α and IL-1 β and ebb of anti-inflammatory factor IL-10. Expression of β-defensins1 ( rBD-1 ) was slight and expression of β-defensins2 ( rBD-2) decreased,which suggested that high glucose or glycosylation reaction probably interfered the constitution expression of rBD-2 and reduced the induction expression. CONCLUSION:There exists chronic inflammatory effect significantly in the diabetic liver. As a key role,the impairment of rBD-2 is probably induced by the high glucose and subsequent glycosylation reaction.
关键词
β-防御素2/糖尿病,实验性/炎症因子/实时定量PCR/大鼠Key words
β-defensin 2/diabetes mellitus,experiment/inflammatory factor/Real-time PCR/rats引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据