NF-κb up-regulates spinal CX3CR1 expression in neuropathic pain rats
AIM: To study the effects of intrathecal infusion of pyrrolidine dithiocarbamate ( PDTC),a nuclear factor-kappa B (NF-κB) inhibitor,on spinal CX3CR1 expression induced by sciatic chronic constriction injury (CCI) and its effects on the development of neuropathic pain in rat. METHODS: Sixty SD rats weighing 230-300 g were randomly divided into S groups ( 12 in each group) : Sham + 9 g/L Normal Saline ( NS ) group,CCI + 9 g/L NS group,Sham + PDTC 1000 pmol/d group,CCI + PDTC 100 pmol/d group and CCI + PDTC 1000 pmol/d group. After implanting intrathecal catheter for 5 d,PDTC or NS was intrathecally administrated once per day. One day later,CCI or sham operation was done and pain threshold was assessed daily. Rats in each group were sacrificed at 4 d after intrathecal infusion and spinal cord lumbar intumescentia expression of CX3CR1 was assessed by Western blotting. RESULTS:;The pain threshold in CCI group significantly decreased compared with that in sham group 1 to 3 d after CCI and maximally decreased at day 3 after CCI(P<0.05). The pain threshold in PDTC + CCI group significantly increased compared with that in CCI group 1 to 3 d after CCI(P<0. 05). The expression of CX3CR1 in PDTC + CCI group significantly decreased compared with those in CCI group ( P < 0. 01 ). CONCLUSION: Intrathecal infusion of PDTC suppresses the spinal CX3CR1 expression and atteruates CCI-induced neuropathic pain in rats. The activated NF-κB pathway may get involved in the development of neuropathic pain through up-regulating spinal CX3CR1 expression.
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万方数据
维普
