Der p2重组BCG对哮喘小鼠气道炎症的抑制作用
Suppression of airway inflammation in a mouse model of asthma by Der p2 recombined BCG
欧阳海峰 1史皆然 1刘阿茹 1遆新雨 1宋立强 1吴昌归1
作者信息
- 1. 第四军医大学西京医院呼吸内科,陕西,西安,710033
- 折叠
摘要
目的:观察胞壁型屋尘螨抗原Der p2重组卡介苗(Der p2 rBCG)对哮喘小鼠气道炎症的抑制作用.方法:实验分4组,小鼠分别给予生理盐水、BCG或Der p2 rBCG处理,然后以Der p2为变应原建立哮喘模型,正常对照组给予生理盐水致敏及气道内滴入,取肺泡灌洗液、肺组织标本,观察肺部炎症细胞浸润、气道杯状细胞增殖及肥大细胞脱颗粒情况.结果:①Der p2 rBCG下调了哮喘小鼠气道局部炎症;②Derp2 rBCG减轻了哮喘小鼠肺组织中的炎症细胞浸润;③Derp2 rBCG可以减轻哮喘小鼠气道粘液高分泌现象并下调杯状细胞数量;④Der p2 rBCG减轻了哮喘小鼠气道中的肥大细胞脱颗粒现象.结论:Der P2 rBCG是一种有效的哮喘免疫治疗方法.
Abstract
AIM: To observe whether Der p2 rBCG suppress ES the airway inflammation in a mouse model of asthma. METHODS: Four groups of mice were vaccinated with PS,BCG or rBCG and were sensitized and challenged with Der p2. Bronchoalveolar lavage fluid and lung tissue were then obtained.The infiltration of inflammatory cells,goblet cell proliferation and mast cell degranulation were observed. RESULTS: Airway inflammation was downregu-lated by Der p2 rBCG in a mouse model of asthma. The infiltration of inflammatory cells in lung tissue was downregulated by Der p2 rBCG. The production of mucus and the number of goblet cells was decreased by Der p2 rBCG. Mast cell mediator release was reduced by Der p2 rBCG. CONCLUSION: Der p2 rBCG may be an efficient immunotherapy for asthma.
关键词
卡介苗/尘螨蛋白2组/哮喘Key words
bacillus calmette guerin/dermatophagoides protein Ⅱ group/asthma引用本文复制引用
基金项目
国家自然科学基金面上项目(30570804)
出版年
2009
NETL
NSTL
维普
万方数据