Effect of curcumine on expression of α-smooth actin and airway remodeling in asthmatic mice
AIM: To investigate the influence of curcumine on α-smooth actin ( SMA) expression and to explore the role of curcumine in asthmatic mice. METHODS: Thirty-six male mice were randomly divided into normal control,experimented group and curcumine treatment group,12 mice in each group. The mice were sensitized and challenged with ovalbumin to establish the asthmatic model. Bronchoalveolar lavage fluid was collected and the number of white blood cell and eosinophilic granulocyte (EOS) was counted. Airway inflammation and alteration of airway structure were detected by HE staining and α-SMA expression in pulmonary tissues was detected by immunohisto-chemistry. RESULTS: The counts of EOS in normal control,experimented group and curcumine treatment group were(0.74 ± 0.26),(5.32 ±0.41) and (3.55 ±0.46) × 10 8/L respectively. Experimented group was significantly higher than normal control group (P<0.01). Airway inflammation was extensive in experimented group. Prominent proliferation of airway epithelial cells,thickness of smooth muscle,hyperplasia of connective tissue,and a marked increase in airway mucus secretion with mucus plugging were observed in experimented group. A great deal of collagen deposition was found in the bronchus submucosa,the afferent wall and surroundings in experiment group. The inflammation was significantly decreased in curcumine treatment group,with decreased production of mucus,decreased collagen and granule of mucus aroundairway,and less proliferation of airway epithelium,smooth muscle hypertrophy and airway spasm. The expression of α-SMA in control group,sensitized group and asthma group were 8. 34% (1/12),58. 33% (7/12) and 33. 33% ( 4/12) respectively. There was statistical significance between the groups( P < 0.01). CONCLUSION: Curcumine inhibits the expression of α-SMA,thus relieving airway inflammation and delaying the process of airwayremodeling.
NETL
NSTL
万方数据
维普
