目的:研究依达拉奉(3-甲基-1-苯基-2-吡唑啉-5-酮,Edaravone)对大鼠弥漫性脑创伤神经功能损伤的治疗作用及其机制.方法:将148只雄性SD大鼠随机分为对照组、创伤组、Edaravone治疗组.采用Marmarou's法建立大鼠弥漫性颅脑损伤模型,于术后6,24 h取大脑皮层冠状逢左右0.2 cm组织脑组织作电镜观察,术后2,4,48,72 h对大鼠神经运动功能和综合运动能力评分.结果:Edaravone治疗组大鼠死亡率(23%)明显低于创伤组(51%,P＜0.05);电镜下,伤后6,24 h Edaravone治疗组中神经元内细胞器、轴索及毛细血管等超微结构的损伤程度明显低于创伤组;神经功能与综合运动能力评分在创伤组中(8.7±1.4,63.8±27.7)明显低于对照组(24.0±0.0,278.0±28.0);Edaravone治疗组(17.4±1.6,118.0±21.0)显著回升(P＜0.05).结论:Edaravone可改善脑创伤后神经功能损伤,其机制与减轻脑创伤后脑组织超微结构损害有关.
Effect of Edaravone on neuromotor function and ultrastructure of brain tissue after traumatic brain injury in rats
AIM: To study the effects of Edaravone on neuromotor function and its mechanism after traumatic brain injury. METHODS: A total of 148 male Sprague-Dawley rats were randomly divided into 3 groups: sham operation group ( n = 33 ),traumatic group(n =70) and Edaravone treatment group(n = 45). DBI rat model was established according to the description of Marmarou's diffused brain injury. The histopathologic changes of cerebral tissue (0.2 cm Bilateralis coronal line) were observed by electron microscope at 6 and 24 h after injury. Behavioral tests were performed daily at 24,48 and 72 h time points. RESULTS: After trauma,the mortality of rats was about 51% in traumatic group and 23 % in Edaravone treatment group. Electron microscope examination showed that the damage of neurons,axons and capillaries in Edaravone treatment group was obviously milder than that in traumatic group. The neu-roscores and the general movement ability in traumatic group (8.7 ±1.4,63.8 ±27.7) were lower than those in sham operation group( 24.0 ±0.0,278.0 ±28.0,P<0.05),while those in Edaravone treatment group(17.4 ±1.6,118. 0± 21.0) were restored markedly(P< 0.05). CONCLUSION: Edaravone dramatically improves neural function after traumatic brain injury and the molecular mechanism is related to the attenuation of traumatic cerebral ulrrastructure damage following tramatic brain.
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