Effect of local temperature change on apoptosis of spermatogenic cells after scrotal reconstruction with skin flap
AIM:To explore the temperature change of testicu-lar surface after scrotal reconstruction with skin flap and its effect on apoptosis of spermatogenic cells. METHODS: Twenty-four male New Zealand albino rabbits were randomly divided into reconstruction group (the group of scrotum reconstruction with flaps) and control group,with 12 rabbits in each group. Twelve female rabbits were used for mating experiment. The sperms were examined and the testicular surface temperature was measured in reconstruction group and the flap reconstruction scrotum models were then established. The data of sperm and temperature were obtained again in reconstruction group in the 8 th weeks after scrotal reconstruction. The testes biopsy was performed randomly for 6 rabbits in experiment group and in control group respectively. The apoptosis of spermatogenic cells was detected by TUNEL assay. In the 8th week after the scrotal reconstruction,matched-pair feed was performed in the other 6 rabbits in reconstruction group and in control group,respectively. RESULTS: The results showed that the testicular surface temperature was higher and the sperm count was lower significantly in reconstruction group than those in control group( P < 0.05 ). The apoptotic index of spermatogenic cells in reconstruction group was significantly higher than that in control group (P < 0.05). In the 8th week after the scrotal reconstruction,male and female matched-pair feed showed infertility in reconstruction group,while (6.0 ± 1. 3) rabbit babies were bom in control group areragely (P<0.05). CONCLUSION: The testicular surface temperature increases after scrotal reconstruction with skin flaps and it leads to spermatogenic cell apoptosis and infertility. Skin flaps are not recommended for reconstruction of the scrotum.
NETL
NSTL
万方数据
维普
