尿道下裂患儿包皮HoxA13基因表达及其病理价值
HoxA13 gene expression and its pathological value in prepuce of patients with hypospadias
熊晶 1魏光辉 1刘星 1吴盛德 1徐明灯1
作者信息
- 1. 重庆医科大学附属儿童医院泌尿外科,重庆,400014
- 折叠
摘要
目的:研究尿道下裂患儿包皮HoxA13核酸及其蛋白表达,探讨其与尿道下裂发生是否具有相关性.方法:以25例尿道下裂及15例包茎患儿为研究对象,选取其包皮组织,采用实时荧光定量PCR及免疫组织化学方法分别检测HoxAl3 mRNA及蛋白表达水平.结果:HoxA13 mBNA表达水平在尿道下裂患儿中明显低于对照组,相对表达量分别为[(9.5±6.5),(52.8±35.7),P＜0.01];HoxA13蛋白表达于皮肤表皮层,在尿道下裂组呈阴性～弱阳性,对照组呈阳性～强阳性.结论:尿道下裂患儿包皮中HoxA13基因及蛋白表达较正常儿童降低,提示HoxA13基因表达异常可能与尿道下裂发生相关,其表达异常可能影响胚胎期尿道发育.
Abstract
AIM: To investigate the etiological role of HoxA13 gene in hypospadias by detecting the HoxA13 gene expression in the prepuce of patients with hypospadias. METHODS: Twenty-five cases with hypospadias and 15 cases with circumcisions (as control) were included in this study. HoxA13 mRNA and protein expression were detected by SYBR green Ⅰ real-time polymerase chain reaction and immunohistochemistry. RESULTS: HoxA13 mRNA expressions in the prepuce of cases with hypospadias reduced significantly compared with those in control [ ( 9.5 ± 6.5 ),(52.8±35.7),P<0.01]. HoxA13 protein expressed in epidermis and localized at cytoplasm.In tissues of hypospadias,the protein showed negative or weakly positive,but strongly positive in the controls. CONCLUSION: HoxA13 expression is significantly lower in the prepuce of cases with hypospadias,indicating that the dysfunction of HoxA13 is possibly related to hypospadias.
关键词
HOX基因/HoxA13基因/尿道下裂/实时荧光定量PCRKey words
HOX gene/HoxA13/hypospadias/SYBR Green Ⅰ real-time PCR引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据