人死亡后特定条件下骨髓细胞核DNA降解与死后时间间隔推断
Relationship between DNA degradation in human marrow nuclei and late postmortem interval after death
罗光华 1靳俊峰 2高翠莲 3王江峰 3陈玉川3
作者信息
- 1. 广东司法警官职业学院司法鉴定系,广东,广州,510520
- 2. 遵义医学院珠海校区,广东,珠海,519041
- 3. 广东警官学院,广东,广州,510232
- 折叠
摘要
目的:探讨人死亡后骨髓细胞核DNA含量变化与晚期死后时间间隔关系.方法:离体人胸骨置于特定条件下,随时间延长分别取材制成病理切片,应用DNA高度特异性染色方法Feulgen改良法结合计算机图像分析技术对死后不同时间胸骨骨髓细胞核DNA含量变化进行分析.结果:人死亡后1～10 d,Feulgen染色阳性产物随死亡时间延长逐渐减少,胸骨骨髓细胞核DNA含量随死亡时间延长呈规律下降,DNA染色逐渐变浅淡,至死后10 d已基本观察不到.结论:人死亡后骨髓细胞核DNA含量随死亡时间延长呈下降趋势,两者之间存在线性关系.
Abstract
AIM: To study the relationship between degradation of DNA in human marrow nuclei and estimation of postmortem interval. METHODS: The amount of nuclear DNA in the marrow of sternum was determined from 1 to 10 d after death stained by improved Feulgen histochemical method with image analysis. RESULTS: The content of marrow DNA decreased gradually with the prolongation of postmortem interval and gradated to its minimum after 10 d after death. CONCLUSION: The content of nuclear DNA in human marrow of sternum reduces with extending time and there is a linear relationship between the degradation rate of the nuclear DNA and postmortem interval.
关键词
死后时间间隔/DNA含量/腐败尸体/胸骨骨髓Key words
postmortem interval/DNA content/corrupt corpse/marrow of sternum引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据