Relationship of dynamic changes between PPARγ and TNF-α and IL-10 in peripheral blood of MODS rats
AIM: To investigate the relationship of dynamic changes between peroxisome proliferator-activated receptor γ, (PPARγ) and TNF-α and IL-10 in peripheral blood of multiple organ dysfunction syndrome (MODS) rats. METHODS: Ninetysix male SD rats were divided randomly into 4 groups: Normal control group, endotoxin (LPS) group, rosiglitazone (ROSI) pretreatment group and PPARγ antagonist CW9662 pretreatment group. Each group was further divided into 4 groups according to different observation time point respectively of 1, 3, 5 and 7 h. Immunocytochemical detection and image analysis were used to detect the PPARγ expression in peripheral blood monanuclear cells. Levels of TNF-α and IL-10 were detected by enzyme-linked immunosorbent assay. RESULTS: The levels of PPARγ in each time-related subgroups in ROIS pretreatment group were all higher than those in LPS group ( P < 0. 05 ). The level of PPARγ was positively correlated to TNF-α ( r= - 0. 635, P < 0. 05 ) and negatively correlated to IL-10 (r =0.605,P <0.05). The levels of PPARγ in each time-related subgroups of CW9662 pretreatment group were higher (P <0.05) than those in LPS group. The level of PPARγ was negatively correlated to TNF-α (r = -0. 725 ,P <0.05 ) and positively correlated to IL-10 ( r = 0. 645, P < 0.05 ).The ratio of IL-10/TNF-α in ROSI pretreatment group showed no significant difference ( P > 0. 05 ) compared with that in normal control group. In GW9662 pretreatment group, the ratio of IL - 10/TNF-α was significantly reduced compared with that in LPS group (P < 0.05 ). CONCLUSION : PPARγ exerts some protective effect on MODS rats by the immune adjustment of anti-inflammatory/pro-inflammatory mechanism.
multiple organ failurePPAR garnmatumoraecrosis factor-alphainterleukin-10
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