Effects of curcumin on cultured rat aortic vascular smooth muscle cell proliferation and apoptosis
AIM: To explore the effects of curcumin on the proliferation and apeptosis of rat vascular smooth muscle cells (VSMCs) and the underlying mechanism. METHODS: VSMCs were primarily cultured by tissue explant isolated from the thoracic aorta of SD rats. VSMCs were treated by curcumin with different concentrations. The viability and proliferation of VSMCs were assayed by MTT and flow cytometry was applied to detect the apoptosis of VSMCs. Western blot was used to detect the expression of P21, P53 and Bcl-2 protein. RESULTS: Administration of 7.5-120 μmol/L curcumin for 24 -72 h significantly inhibited the proliferation of VSMCs in a dose and time-dependent manner (P < 0.05). After VSMCs were treated with 30 and 60 μmol/L curcumin for 24 h, flow cytometry demonstrated that the apoptosis rate of the cells significantly increased. Curcumin at 30 μmol/L up-regulated P21waf1/cip1 expression, down-regulated Bcl-2 expression and exerted no obvious influence on the P53 expression at different time points. CONCLUSION: Curcumin inhibits the proliferation and induces the apoptosis of VSMCs, which may relate to the up-regulation of P21 and down-regulation of Bcl-2.
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维普
