Effect of human peritoneal mesothelial cells on adhesion, migration and invasion of ovarian carcinoma SKOV3 cell line
AIM: To explore the effect of human peritoneal mesothelial cells (HPMC) on the adhesion, migration and invasion of ovarian carcinoma SKOV3 cell line and the potential mechanism. METHODS: The primary culture method for HPMC was established. Isolated HPMC were identified and some cell factors expression were examined by immunohistochemical analysis. Adhesion assay, migration assay and invasion assay were used to evaluate the metastatic ability of SKOV3. The role of axis of CXCL12-CXCR4 in SKOV3 metastatic ability was also studied. RESULTS: HPMCs were suecessfully cultured and CXCL12 and MSLN were expressed in HPMC, but CXCR4 was not expressed. In the group of the co-cultured HPMC and SKOV3 cells, the absorbance, the number of migration and invasion cells were signifocantly higher than those in the group of single culture SKOV3, with statistically significant difference ( P < 0.05 ). In the group of co-cultured HPMC and stable and high CXCR4 expression of SKOV3 cells( SKOV3-CXCR4-cDNA), the absorbance, the number of migration and invasion cells were significantly higher than those in the other three groups, with statistically significant difference( P < 0.05 ). CONCLUSION: Interactions of ovarian carcinoma SKOV3 cells and HPMC contribute to the adhesion, migration and invasion of SKOV3 and the mechanism may relate to the axis of CXCL12-CXCR4.
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万方数据
维普
