Differential protein expression of gastric cancer and liver cancer in serum using magnetic bead based sample fractionation and MALDI-TOF-MS
AIM: To establish differential proteintial expression using functional magnetic bead based sample fractionation method combined with matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS) technology and to explore potential biomarkers of gastric cancer and liver cancer for early diagnosis. METHODS: Plasma protein profiling of three groups including 20 gastric cancer patients and 10 liver cancer patients as well as 20 normal controls was analyzed by ClinProt magnetic bead enrichment and MALDI-TOF-MS. RESULTS: Two peaks with mass charge ratio(m/z) 2863 u and 4965 u optimally discriminated gastric cancer from non-cancer control group, m/z 1618 u and 4965 u optimally discriminated liver cancer from non - cancer control group, m/z 4965 u was up-regulated in the two kind of cancers. CONCLUSION: Functional magnetic bead based sample fractionation method combined with MALDI-TOFMS is accurate and stable. Protein peaks with m/z 2863 u, 1618 u and 4965 u may be potential biomarkers for gastric cancer and liver cancer.
NETL
NSTL
万方数据
维普
