应用液体蛋白芯片飞行时间质谱系统研究胃癌、肝癌血清差异蛋白表达
Differential protein expression of gastric cancer and liver cancer in serum using magnetic bead based sample fractionation and MALDI-TOF-MS
李莉 1谌宏鸣 2李甜 2胡晓慧3
作者信息
- 1. 新疆医科大学附属肿瘤医院,新疆,乌鲁木齐,830011
- 2. 新疆医科大学基础学院组织胚胎学教研室,新疆,乌鲁木齐,830054
- 3. 博扬通北京科技有限公司,北京,100080
- 折叠
摘要
目的:研究胃癌、肝癌和正常人的血清差异蛋白表达,寻找具有潜在诊断意义的血清标志物,以利于胃癌、肝癌的早期诊断.方法:收集胃癌患者20例和肝癌患者10例以及正常人20例血清样本,经ClinProt磁珠纯化、基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)分析及ClinPro-Tools生物信息学方法研究其血清蛋白表达谱.结果:质荷比(m/z)为2863 U和4965 u的蛋白峰能较好地鉴别胃癌和非癌组.m/z为1618 U和4965 U的蛋白峰能较好地鉴别肝癌和非癌组.m/z为4965 u的蛋白峰在胃癌、肝癌中较正常人均异常高表达.结论:液体蛋白芯片飞行时间质谱系统作为研究蛋白表达谱的工具,具有较好的重复性与精度,筛选得到的m/z为2863,1618,4965 u的蛋白可能为潜在的胃癌、肝癌肿瘤标志物.
Abstract
AIM: To establish differential proteintial expression using functional magnetic bead based sample fractionation method combined with matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS) technology and to explore potential biomarkers of gastric cancer and liver cancer for early diagnosis. METHODS: Plasma protein profiling of three groups including 20 gastric cancer patients and 10 liver cancer patients as well as 20 normal controls was analyzed by ClinProt magnetic bead enrichment and MALDI-TOF-MS. RESULTS: Two peaks with mass charge ratio(m/z) 2863 u and 4965 u optimally discriminated gastric cancer from non-cancer control group, m/z 1618 u and 4965 u optimally discriminated liver cancer from non - cancer control group, m/z 4965 u was up-regulated in the two kind of cancers. CONCLUSION: Functional magnetic bead based sample fractionation method combined with MALDI-TOFMS is accurate and stable. Protein peaks with m/z 2863 u, 1618 u and 4965 u may be potential biomarkers for gastric cancer and liver cancer.
关键词
胃肿瘤/肝肿瘤/液体蛋白芯片飞行质谱/差异蛋白/早期诊断Key words
gastric neoplasms/liver neoplasms/ClinProt system/differential protein/early diagnosis引用本文复制引用
基金项目
新疆维吾尔自治区高校科研计划重点项目(XJEDU2005115)
出版年
2009
NETL
NSTL
维普
万方数据