Chronic cold stress-induced liver impairment of rats and its mechanism
AIM: To study the impairment of chronic cold stress on rat liver and the involvement of c-Jun N-terminal kinase(JNK) pathway. METHODS: Adult male SD rats were placed in the re-frigerator( 6 h for 1,3,5,7,10 and 14 d,respectively) and the exposure temperature was( - 15 ± 1 )℃. The impairment of liver was observed by HE staining (hematoxylin and eosin stain) and the expression and activation of c-Jun N-terminal kinase ( JNK ) and extracellular signal-regulated kinase (ERK) were determined by Western blot. RESULTS: Cold stress induced significant pathological impairment of the rat liver. Although partially recovered at 7 d,the impairment aggravated again thereafter. Western blot showed that the level of phosphorylated JNK increased after the cold exposure and the increase was significantly correlated to the impairment of the rat liver. But no significant changes were observed in the expression and activation of ERK. CONCLUSION: Cold stress leads to impairment of the rat liver and the increased phosphorylation of JNK may contribute to the cold-induced liver impairment.
cold stressratlivermitogen activated protein kinase (MAPK)
NETL
NSTL
万方数据
维普
