Change of motility in vitro and myosin heavy chain in sphincter of Oddi after cholecystectomy
AIM: To study the alteration of rabbits' sphincter of Oddi after cholecystectomy. METHODS;Sixty adult rabbits were divided into 4 groups(A,B,C and D),with 18 rabbits in A and D,and 12 rabbits in C and D. In the post cholecystectomy groups(B,C and D),the rabbits underwent cholecystectomy and then were bred for 2,4 and 8 weeks respectively,while the rabbits in the control group (A of sham operation) were bred for 8 weeks. Then,the rabbits' sphincters of Oddi were dislodged. Six rabbits of A and D groups were detected by myosin heavy chain (MHC) immunohistochemistry,12 rabbits of the 4 groups were measured by manometer in vitro and MHC expression was examined by western blot. RESULTS: Amplitude of manometer wave increased significantly but not in A group (P <0. 01) and MHC expression in SO of B,C,D groups significantly increased compared with that of A group (P < 0. 01 ). Immunohistochemistry showed that smooth muscle cells of D group were more concentrated than those in A group in the dye of myosin. CONCLUSION: SO is adaptively changed after cholecystectomy,which may be one of the etiopathogenisis for many clinical problems concerning sphinter of Oddi disfunction in postcholecystectomy patients.
postcholecystectomysphincter of oddimotility in vitromyosin heavy chain
NETL
NSTL
万方数据
维普
