Change of neuroglobin in rats during endotoxic shock
AIM:To study the changes and significance of neuroglobin ( Ngb) expression in hippocampus,cerebrospinal fluid (CSF) and plasma in rats under endotoxic shock condition. METHODS: Seventy adult Sprague-Dawley(SD) rats were randomly divided into control group (0.5 mL normal saline was injected via vena caudalis)and experimental groups (0.5 mL endo-toxin was injected via vena caudalis,0.016 g/kg). The brain tissues were examined respectively at the time point of 3,6,12,24,48 and 72 h( n = 10). The expression of Ngb over different periods was measured by enzyme-linked immunpsorbert assay ( ELISA ),Westem-blot and immunohistochemical method (IHC). The brain water content (BWC) was measured by wet and dry weight method. RESULTS: EUSA showed that the Ngb concentrations in the plasma,CSF and hippocampus after endo-toxin injection were significantly higher than those in control group (P < 0.01) and peaked at 48 h,which were 34. 89,22. 01 and 14.01 mg/L respectively. Western Blot showed that the average molecular weight (Mr) of Ngb in each group was 17 × 103. The quantity of Ngb expression in the intervention groups was higher than that in the control group. The expression in 3 h group was the lowest and the expression in 48 h group was the highest. IHC showed that Ngb-immunoreactive product was located in the cytoplasm of the pyramidal neurons and was yellow-brown in color. The BWC in each group after endotoxin injection significantly in-creased( P < 0. 01) and peaked at 48 h. The peak proportion of BWC was 0.8313. CONCLUSION: Our results indicate that there is a positive correlation between the up-regulation of Ngb ex- pression and the severity of brain impairment. The up-regulation of Ngb expression might be one of the endogenous nuroprotective mechanisms under endotoxic shock condition.
NETL
NSTL
万方数据
维普
