Relation between inflammation and growth and metastasis of Lewis lung carcinoma in process of serial passage in mice
AIM: To observe the changes of neoplastic growth and metastasis and the variation of inflammation in neoplasm in the serial passage of tumor-bearing mice and to discuss the correlation between the variations of tumor biological characters in vivo and inflammation. METHODS: Lewis lung tumor cells were inoculated subcutaneously into C57/BL6 mice and the first generation of tumor bearing mice was established. The tumor bearing mice were then serially passaged. Forty-five mice were inoculated on the second generation (the early group),the eighth generation (the intermediate group) and the fifteenth generation (the late group),with 15 mice in each group. To observe the time of onco-genesis and the rate of pulmonary metastasis,immunohistochemis-try was used to detect the density of macrophages,the microvessel density (MVD),the expressions of nuclear factor-kappa B ( NF-κB ) and matrix metalloproteinase-9 ( MMP-9) in neoplasm in each group. RESULTS: Oncogenesis was speeded up in the 3 groups with the increasing times of passages( P < 0.01). The rate of lung metastasis increased significantly ( P < 0. 01 ) and the rate of pulmonary metastasis in the early,intermediate and late groups was 13.3%,60% and 100%,respectively. The density of mae-rophage and the microvessel density (MVD) in the 3 groups was respectively (24.87 ± 8. 54),(36. 33 ± 10. 02) and (47. 20 ± 13.49),and (24.73 ± 10.38),(41.27 ±13. 50)and (55.80± 21.31),with significant difference (P < 0. 01,both). The expression of NF-κB and MMP-9 in neoplasm gradually increased (P<0. 01) and the expression of CD31,NF-κB and MMP-9 were positively correlated to the density of macrophage in peo-plasm( P < 0. 01). CONCLUSION: The growth and metastasis of Lewis lung carcinoma gradually enhance in the process of serial passage in miee and the dynamic variations are correlated with the inflammation in neoplasm.
NETL
NSTL
万方数据
维普
