膜联蛋白5在大鼠消化器官中的细胞定位
Cellular localization of annexin 5 in rat's digestive organs
张艳梅 1韩雪峰 1卢坤刚 1陶晓倩 1柳海燕 1时姗姗 1姚兵2
作者信息
- 1. 南京师范大学生命科学学院,江苏,南京,210046
- 2. 南京师范大学生命科学学院,江苏,南京,210046;南京军区南京总医院解放军临床检验医学研究所,江苏,南京,210002
- 折叠
摘要
目的:观察膜联蛋白5(Annexin 5)在大鼠部分消化器官中的细胞定位,为探讨Annexin 5在消化器官内可能发挥的生理学作用奠定基础.方法:选取健康SD大鼠,经Bouin's液固定其消化器官(胃、小肠、肝脏、胰腺)后,应用特异的兔抗Annexin 5抗血清,采用免疫组织化学方法和免疫荧光化学方法,观察Annexin 5在大鼠消化器官中的细胞定位.结果:Annexin 5在大鼠的消化器官中呈细胞特异性分布.Annexin 5在胃上皮细胞、胃底腺部分细胞、小肠绒毛杯状细胞、胆管内皮细胞和血管内皮细胞中分布较多,同时在胃和肠的黏膜层和肌层的部分细胞、小肠固有层中某些细胞、肝内皮细胞、胰腺血管内皮细胞中也有分布.结论:Annexin 5存在于大鼠消化器官执行特殊功能的一些细胞内,提示Annexin 5可能在这些细胞发挥生理功能的过程中具有特定的作用.
Abstract
AIM:To investigate the cellular distribution of annexin 5 in rat's digestive organs using immunohistochemistry and immunofluorescence. METHODS: Digestive organs from healthy rats were fixed with Bouin's solution. Annexin 5 was examined for its specific distribution in rat digestive organs by immunohistochemistry and immunofluorescence with specific antiserum to rat annexin 5. RESULTS: Annexin 5 was localized in digestive organs of rats in specific cell types. Annexin 5 mainly existed in epithelial cells in stomach,partial cells in fundus glands,goblet cells in small intestine,endothelial cells in bile duct and endothe-lial cells in blood vessel of liver. Annexin 5 was also observed in mucous membrane and muscular layer of stomach and small intestine,lamina propria cells in small intestine,endothelial cells in liver and endothelial cells in blood vessel in pancreas. CONCLUSION: The existence of annexin 5 localized in many cell types with specific function in rat's digestive organs indicates that annexin 5 might be involved in these physiologic functions.
关键词
膜联蛋白5/消化器官/定位Key words
annexin 5/digestive organs/localization引用本文复制引用
基金项目
国家自然科学基金(30770801)
江苏省自然科学基金(BK2006135)
出版年
2009
NETL
NSTL
维普
万方数据