Construction and expression of CbpA based-DNA vaccine of Streptococcus pneumoniae and evaluation of its protection efficacy
AIM: To construct a recombinant eukaryotic expression plasmid pcDNA3.1/CbpA and to detect its protective effect against invasive pneumocoecal infection,METHODS: CbpA gene was amplified from Streptococcus peumoniae TIGR4 by PCR and was then inserted into the eukaryotic expression vector pcD-NA3.1 ( + )to construct recombinant pcDNA3.1/CbpA. Western blot was used to detect the protein expression of the CbpA gene in transfected COS-7 cells. The recombinant pcDNA3. 1/CbpA was injected into BALB/C mice via an intramuscular route to examine its capacity of protecting mice against intraperitoneal challenged with TIGR4. RESULTS: pcDNA3. 1/CbpA recombinant plasmid was successfully constructed and cbpA protein was expressed in transfected COS-7 cells. Antigen-specific antibody was detected in mice immunized with pcDNA3. 1/CbpA. Protection assay demonstrated that the survival rate of the mice immunized with pcDNA3. 1/CbpA was higher than that of control group. CONCLUSION: pcDNA3. 1/CbpA induces protective immune response in mice and could be used as a potential vaccine to prevent pneumococcal infection.
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NSTL
万方数据
维普
