Differentiation of mouse embryonic stem cells into cardiomyocytes in vitro
AIM: To investigate a method of inducing embryonic stem cells ( ESCs ) of mouse high differentiation into eardiomyo-cytes in vitro. METHODS:Six groups were set up according to inducers added in the culture system of hanging drop culture and suspension culture respectively: group Ⅰ ( salvia miltorrhiza and 5-azacytidine as inducer),group Ⅱ (salvia miltorrhiza and retino-ic acid as inducer),group Ⅲ (5-azacytidine as inducer),group Ⅳ (salvia miltorrhiza as inducer),group Ⅴ (retinoic acid as inducer) and group Ⅵ ( no inducer). The rates of differentiation of cardiomyocytes from ESCs were compared between the 6 groups using respectively the two culture systems. RESULTS: The rates of differentiation of group Ⅰ,Ⅲ,Ⅳ,Ⅴ and Ⅵ were (77.78 ± 5.09)%,(66.67 ±8. 82)%,(51. 11 ± 1. 92)%,(44.44 ± 5.09)% and (23. 33 ± 3. 34)% in hanging drop culture and (42.22 ±6.94)%,(36.67 ±8. 82)%,(25.55 ± 10.71)%,(17.78±5.09)% and (10.00 ±2.84)% in suspension culture,with statistical difference between the two methods (P < 0. 05). The rate of differentiation of the group Ⅰ was the highest among the 6 groups (P <0.05) by hanging drop and as high as 78% of the beating embryoid bodies (EBs)were found. CONCLUSION: The application of traditional Chinese medicinal herb salvia miltorrhiza plus 5-azacytidine as inducers in culture system of hanging drop cultures achieves higher rate of differentiation of cardiomyocytes derived from ESCs in vitro.
NETL
NSTL
万方数据
维普
