不同遗传背景及增龄对大鼠高血压胰岛素抵抗相关基因表达谱的影响
Effect of aging and different genetic background on insulin resistance related genes expression in rats
田红燕 1万红梅 1马爱群 1韩克 1王亭忠 1胡志 1席雨涛 1范粉灵1
作者信息
- 1. 西安交通大学第一附属医院心内科,陕西 西安 710061
- 折叠
摘要
目的:探讨不同遗传背景以及增龄对大鼠高血压胰岛素抵抗相关基因表达谱的影响,筛选高血压胰岛素抵抗相关基因.方法:以自发性高血压(SHR)大鼠和Wistar-Kyoto(WKY)大鼠为动物模型,用S4000点基因表达谱芯片分别检测不同遗传背景(14 wk SHR大鼠和14 wk WKY大鼠)的大鼠肾组织差异表达基因、增龄(SHR大鼠从5 wk增龄至14wk)对SHR大鼠肾组织基因表达谱的影响,并从中随机选取2个差异表达基因,用RT-PCR方法进行验证.结果:①SHR大鼠增龄后肾组织中差异表达基因209个;14 wk SHR大鼠与14 wk WKY大鼠相比较肾组织差异表达基因有166个;②根据SHR大鼠增龄及不同遗传背景大鼠肾组织2种基因表达谱芯片检测结果显示,两者共同差异表达基因11个,其中免疫相关基因2个、代谢相关基因4个、其他类型基因5个.结论:代谢、免疫相关基因表达差异可能在高血压胰岛素抵抗发病中起重要作用.
Abstract
AIM: To explore the effect of aging and different genetic background on the insulin resistance related genes expression in rats and to screen the closely relative genes of the hypertension with insulin resistance. METHODS: Spontaneously hypertensive rats(SHR) and Wistar-Kyoto( WKY) rats were used as models and S4000 point gene-chip was used to detect the gene differential expression of kidney tissues in rats with different genetic back-ground( 14 wk SHR and 14 wk WKY). The effect of aging(5 wk to 14 wk SHR) on the gene expression profile was also analyzed. RESULTS: A total of 209 differential genes expression in kidney tissues were found in rats with the different ages (5 wk SHR and 14 wk SHR),and 166 differential genes expressions were found in rats with different genetic background (14 wk SHR and 14 wk WKY),Results of gene-chip detection showed that there were 11 common differential expression genes in the two groups,including 2 immunity related genes and 4 energy metabolism related genes. CONCLUSION: The genes differential expression related to metabolism and immunity may play an important role in the onset of hypertension and insulin resistance.
关键词
高血压/基因芯片/胰岛素抵抗Key words
hypertension/gene chip/insulin resistance引用本文复制引用
基金项目
美国中华医学基金会资助项目(2002-2007)
出版年
2009
NETL
NSTL
维普
万方数据