Inhibitory effects of oxymatrine-carbenoxolonesodium inclusion complex on central nervous system in mice
AIM: To explore the influence of oxymatrine-carben-oxolone sodium inclusion complex ( OCSIC ) on the central nervous system. METHODS: The action of OCSIC on central nervous system was studied by observing the changes of initiative and passive activity's tolerance and harmonious motion,by measuring the influence of pentobarbital's hypnotic action,by coordinating with GABA,Diazepam or Ethylnipecotate and antagonizing the convulsion caused by Picrotoxin,Kainic acid and N-methyl-D-aspartate,and by adopting immunohistochemistry method ( SABC) to determine the gamma aminobutyric acid transporter-1 Protein (GAT-1) expression in the cerebral cortex and hippocampi in mice. RESULTS: Compared with those in control group,the spontaneous activity were reduced to 86. 7%,85. 6% and 17. 2% ( P < 0.01),the latent period of falling asleep induced by pentobarbital administration was shortened to 61. 2%,49. 0% and 36. 7% (P< 0. 01 ),the sustained period of sleep was lengthened to 379.8%,146.2% and 132.2% (P<0. 01,P<0.05),and the hypnotic action subsequent to the administration of pentobarbital in a subthreshold dose was greatly enhanced ( P < 0. 05) in mice treated intraperitoneally with respective OCSIC 100,50 or 25 mg/ kg in experimental group. OCSIC alone in a subthreshold dose reduced spontaneous activity to 78. 8% and combined administration with GABA or with Ethyl nipecotate in a subthreshold dose reduced spontaneous activity to 47. 0% (P < 0. 01 ). OCSIC 100 mg/kg with a subthreshold dose of anticonvulsion Diazepam (0.5 mg/kg) antagonized the convulsion caused by Picrotoxin ( P < 0.05),but not antagonize the convulsion caused by Kainic acid and N-methyl-D-aspartate. OCSIC decreased the number of GAT-1 in the cerebral cortex and hippocampi of mice ( P < 0. 01 ). CONCLUSION: OCSIC exerts inhibitory effects on the central nervous system,which may be related with GABA.
NETL
NSTL
万方数据
维普
