Chromosome aberration during malignant transformation of human bronchial epithelial cells induced by cigarette smoke condensate
AIM:To study the relationship between chromosome aberration and malignant transformation of immortalized human bronchial epithelial ( BEP-2D) cells and to explore the mechanisms of smoking-induced lung cancer. METHODS: Cells were divided into control group and experimental group,with immortalized human bronchial epithelial (BEP-2D)cells and BEP-2D cells (including P15,P20,P25,P30,P35 and P38 cells)treated by cigarette smoke condensate ( CSC),respectively. Cytogenetics methods were used to analyze the chromosome aberration of BEP-2D cells treated by CSC. RESULTS: The cells in control group kept a relatively stable pattern of karyotypes and chromosome numbers,with a ratio of 94% -98% for the cells of diploid and with 17 (3%,in percentage) cells of aneuploid and polyploidy. The cells in experimental group gradually lost their normal diploid karyotypes (beginning from P15 cells). The ratio of diploid cells decreased from 83% to 53% and the number of aneuploid and polyploidy cells was 196 (32.7%,in percentage),with statistically significant difference ( χ2 = 152. 5552,P < 0. 01 ). The number of cells with chromosome aberration was 6 (1 % in per-centage)and 60 (10% in percentage) respectively in control group and in experimental group,with statistically significant difference (χ2 =40.0834,P <0.01). Additionally,the phenomena of en-doradiosonde and circular chromosome was found in P38 cells in experimental group,with a ratio of 4% and 1%,respectively. CONCLUSION: Cigarette smoke condensate may result in the instability of chromosomes of BEP-2D cells,with an inducement of aberration and malignant transformation. The chromosome aberration,found in the P15 cells,could be taken as an early event in the process of malignant transformation of BEP-2D cells.
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