Bone marrow-derived dendritic cells from traumatized mice display reduced capacity of inducing T cells responses
AIM: To study the capacity of bone marrow-derived dendritic cells( BMDCs) in inducing allogeneic T cells responses after hemorrhage combined with closed femur fracture in mice. METHODS;Bone marrow was isolated 24 h after hemorrhage combined with closed femur fracture. BMDCs were induced in vitro by recombinant murine granulocyte/macrophage-colony stimulating factor ( rmGM-CSF). The capacity of immature DC and mature DC in inducing allogeneic T cells responses was determined by mixed lymphocyte reaction (MLR),the expressions of major histocompatibility complex class Ⅱ ( MHC Ⅱ) and costimu-latory molecules including CD40,CD80 and CD86 on DC surface were measured using flow cytometry and the levels of interleukin-12(IL-12) p40,IL-12p70 and interleukin-10(IL-10) in lipopo-lysaccharide( LPS) -stimulated DC supernatants were detected by enzyme-linked immunosorbent assay (ELISA ) kits. RESULTS: BMDCs from traumatized mice,regardless of LPS-induced maturation,mediated significantly reduced MLR in comparison with normal controls (P <0,05). CD40 expression on BMDCs from traumatized mice,before or after LPS stimulation,was significantly lower than that in normal controls [(4.0 ± 1. 0) % vs (22.0± 3.5)%;(56.0 ±7.5)% vs (91.0 ±8. 0% ),P <0.01 ],but no significant changes of MHC Ⅱ,CD80 and CD86 expression on BMDCs were observed between the two groups. The levels of IL- 12 p40 and IL-12p70 in LPS-stimulated BMDCs supernatants from traumatized miee were lower than those in normal controls [ (45.0 ± 6.5) vs (78.0±6.8)ng/L;(9.0±l.0)vs (18.0±1.9)ng/L,P < 0.05 ] while no significant difference IL-10 level was observed between the two groups. CONCLUSION: BMDCs from traumatized mice are of reduced capacity in inducing T cells responses,which may be partially attributable to the decreased expression of costimulatory molecule CD40 and inadequate IL-12 secretion.
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万方数据
维普
