Study on abnormality of PTEN gene and its expression in human gliomas
AIM:To study the deletion and mutation of the exon 7 of PTEN gene and the expression of PTEN protein in human gloimas and to analyze the relationships between PTEN and clinical classifications. METHODS: Included in the study were 64 cases;29 grade Ⅰ and Ⅱ and 35 grade Ⅲ and Ⅳ. Exon 7 of PTEN gene was examined for its deletion by differential polymer-ase chain reaction (DPCR) and its mutation by single-strand conformation polymorphism( SSCP) with silver staining and DNA sequencing. The expression of the PTEN protein was measured by immunohistochemistry. RESULTS: The whole deletion and mutation of exon 7 of PTEN gene in gliomas was 51.56%,with 14% in grade Ⅰ and Ⅱ,and 83% in grade Ⅲ and Ⅳ (P <0. 05). The expression of PTEN protein in gliomas was 48. 44%,with 56% in grade Ⅰ and Ⅱ,and 34% in grade Ⅲ and Ⅳ (P<0.05). The positive expression of PTEN protein in abnormal group ( 97 % ) was lower than that in the normal group (3 % ) with a significant difference between them ( P < 0. 05). CONCLUSION: PTEN gene deletion and mutation is a late occurrence of glioma genesis and the abnormal PTEN gene promotes the malignant progress of glioma. The positive expression of PTEN reduces to some extent with the increase of glioma malignancy. PTEN gene deletion and mutation is negatively correlative to its positive expression. The abnormal PTEN gene influences the progress of transcription and translation in the course of PTEN protein synthesis.
NETL
NSTL
万方数据
维普
