Investigation of differential gene expression profiles in mice cryptorchid testes by cDNA microarray
AIM:To study the differential gene expression profiles between the normal and cryptorchid mice testes by cDNA microarray. METHODS: BLAC mice were unilaterally produced cryptorchidism artificially. Four weeks later,we extracted mRNA from both normal and cryptorchid testes,produced cDN A probes and employed them on cDNA microarray to detect the differential gene expression profiles. RNA dot blotting was taken on COX8a,a down-expressed gene in the previous hybridization,to validate the microarrays. RESULTS: Thirty-four differential expressed genes related to cryptorchism were found. CONCLUSION: Screening of the differential gene expression profiles between the normal and cryptorchid mice testes by cDNA microarray can be used in the study of cryptorchid-related genes and further researches. COX8a may play a role in the development and progression of cryptorchidism.
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NSTL
万方数据
维普
