脑出血大鼠细胞凋亡与缺氧诱导因子HIF-1α的相关性及促红细胞生成素对其影响
Experimental study on effect of rhEPO on brain edema,HIF-1α and apoptosis in rats with intracerebral hemorrhage
杜志刚 1赵宝玲 1王艳玲 2伊红丽 2何山松 1王志刚1
作者信息
- 1. 北京市通州区第二医院神经内科,北京,101102
- 2. 华北煤炭医学院附属医院神经内科,河北,唐山,063000
- 折叠
摘要
目的:研究脑出血大鼠细胞凋亡与缺氧诱导因子HIF-1α的相关性以及重组人促红细胞生成素(rhEPO)对脑出血后脑水肿及缺氧诱导因子的影响.方法:采用自体血脑内注射法建立脑出血动物模型,应用免疫组化方法检测脑出血后不同时间血肿周边脑组织中HIF-1α蛋白表达情况,并应用干湿比重法测定脑组织含水量,应用TUNEL法测凋亡细胞.结果:出血后,HIF-1α蛋白阳性表达率随时间进行性增加,并于出血后72 h达到高峰且维持到第7日,此后HIF-1α蛋白表达阳性率逐渐下降,脑出血后不同时间阳性率比较具有显著性差异(P＜0.01);脑出血后3 d内,脑组织含水量进行性增加,此后逐渐下降,脑出血EPO干预组较脑出血组及对照组各时间点脑组织含水量降低,HIF-1α蛋白阳性率减少.结论:缺血半暗带区脑组织中HIF-1α与细胞凋亡和脑水肿表达具有相关性.rhEPO能显著降低脑出血后脑组织含水量,减少HIF-1α表达,对脑出血后脑组织有良好的保护作用.
Abstract
AIM: To explore the effect of rhEPO on hif-1 and brain edema in rats with intracerebral hemorrhage ( ICH). METHODS: One hundred rats were randomly divided into pseudooperation group and groups of 3,6,12,24,48,72 h and 7,14 and 21 d after ICH (n =5,each group). The model of ICH was established in rate by intracerebral injection of autogenous blood. The expressions of HIF-1α in rat tissue around the hemorrhagic and the normal brain tissue were detected with SP im-munohistochemical method. The expression rate of HIF-la was compared with the degree of brain edema at different time points after ICH. TUNEL method was used to detect apoptosis. RESULTS: The water content in rhEPO group was lower than that in intracerebral hemorrhage groups. The rate of hif-1 in rhEPO group was lower than that in intracerebral hemorrhage groups. CONCLUSION: Apoptosis in rat brain tissue around the damaged areas after ICH is associated with activation of hypoxia inducible factor-1α and brain edema. rhEPO significantly reduces brain edema and the rate of the hif-1,thus protecting the brain after intracerebral hemorrhage.
关键词
红细胞生成素/缺氧诱导因子1/细胞凋亡/脑水肿/脑出血Key words
erythropoietin/cerebral hemorrhage/apoptosis/HIF-1/brain edema引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据