腺病毒介导canstatin基因对肝癌的抑制作用
Anti-tumor effect of canstatin gene on liver cancer in vivo
亓民 1李建生 1马军 2李印3
作者信息
- 1. 第一附属医院消化内科,河南,郑州,450052
- 2. 第二附属医院消化内科,河南,郑州,450014
- 3. 河南省肿瘤医院胸外科,河南,郑州,450003
- 折叠
摘要
目的:探讨人canstatin基因对人肝癌移植瘤模型的抑制作用.方法:应用人肝癌细胞株SMMC-7721建立移植瘤模型.将裸鼠随机分成PBS组、空载体组和载体治疗组(腺病毒载体AdCan),两次注射.治疗期间测量皮下移植瘤的长径和短径;30 d后处死裸鼠,取肿瘤行常规病理切片,观察肿瘤组织坏死情况;检测肿瘤组织中caspase-3,Flk-1的表达.结果:完成注射治疗后第3日,AdCan基因治疗组肿瘤体积显著小于其余两组;治疗期间,HE染色显示AdCan基因治疗组坏死最明显;AdCan基因治疗组caspase-3表达高于空载体组和对照组;canstatin基因治疗组Flk-1的表达低于空载体组和对照组.结论:人canstatin基因对肝癌移植瘤的生长具有抑制作用,作用机制可能是降低Flk-1的表达进而抑制肿瘤的血管生成,抑制肿瘤的生长.
Abstract
AIM:To investigate the anti-tumor effects of canstatin gene on human liver carcinoma(SMMC-7721). METHODS: Tumor xenografts were induced by injection of 1 × 107 SMMC-7721 cells into the left axilla of BALB/c nude mice. The animals were randomized into 3 groups ( Adcan,AdGFP and PBS). During the treatment period,the size of tumors was measured with caliper every 3 d. At the end of the experiment( day 30),all the tumors were resected and both routine pathology and immunohisto-chemical examinations were performed to observe the drug toxicity and the expression of Flk-1 and caspase-3. RESULTS: In canstatin group,the tumors were significantly suppressed in comparison with those in control groups. The HE staining showed many large necrotic regions in tumors from each group,especially in the Ad-canstatin-treated group. The expression of Flk-1 in tumor cell membrane decreased in mice treated with canstatin. The expression of caspase-3 in tumor cell membrane increased in mice treated with canstatin. CONCLUSION: Canstatin inhibits the growth of human primary liver carcinoma by restraining the angiogenesis of tumors.
关键词
基因疗法/canstatin/半胱氨酸天冬氨酸蛋白酶-3/Flk-1/癌,肝细胞Key words
gene therapy/canstatin/caspase-3/Flk-1/carcinoma,hepatocellular引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据