Anti-tumor effect of canstatin gene on liver cancer in vivo
AIM:To investigate the anti-tumor effects of canstatin gene on human liver carcinoma(SMMC-7721). METHODS: Tumor xenografts were induced by injection of 1 × 107 SMMC-7721 cells into the left axilla of BALB/c nude mice. The animals were randomized into 3 groups ( Adcan,AdGFP and PBS). During the treatment period,the size of tumors was measured with caliper every 3 d. At the end of the experiment( day 30),all the tumors were resected and both routine pathology and immunohisto-chemical examinations were performed to observe the drug toxicity and the expression of Flk-1 and caspase-3. RESULTS: In canstatin group,the tumors were significantly suppressed in comparison with those in control groups. The HE staining showed many large necrotic regions in tumors from each group,especially in the Ad-canstatin-treated group. The expression of Flk-1 in tumor cell membrane decreased in mice treated with canstatin. The expression of caspase-3 in tumor cell membrane increased in mice treated with canstatin. CONCLUSION: Canstatin inhibits the growth of human primary liver carcinoma by restraining the angiogenesis of tumors.
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