目的:观察NDRG2(N-myc downstream regulated gene 2)对于乳腺癌细胞系增殖的影响.方法:采用脂质体转染法将NDRG2基因转入乳腺癌细胞系SK-BR-3,经G418筛选后获得稳定高表达NDRG2的亚克隆细胞系SK-BR-3/NDRG2-flag 及仅转染载体的SK-BR-3/pcDNA3.1(+).应用Western Blot 检测NDRG2在转染细胞中的表达;应用平板克隆形成试验、MTT、流式细胞仪检测转染前后细胞增殖能力的差异.结果:成功建立了稳定高表达NDRG2的亚克隆细胞系SK-BR-3/NDRG2-flag;NDRG2的高表达导致了乳腺癌细胞系增殖能力减弱(P＜0.01);细胞周期阻滞在G0/G1期.结论:在乳腺癌细胞系SK-BR-3中,过表达NDRG2可有效抑制细胞增殖并导致细胞周期的阻滞.
Effect of NDRG2 on proliferation of breast cancer cells
AIM:To explore the effect of gene NDRG2 (N-myc downstream regulated gene 2) transfection on the proliferation and apoptosis in breast cancer cells. METHODS: pcDNA3. 1-NDRG2-flag and eukaryotic expression vectors pcDNA3. 1 ( + ) were transfected into breast cancer cell line SK-BR-3 by induction of liposome. The subclone cell lines SK-BR-3/NDRG2-flag expressing NDRG2 stably and highly were obtained by persistent G418 selection. NDRG2 expression levels in the transfected cell lines were detected by Western blot and the proliferation activity of the cell lines was detected by plate colony formation,MTT and flow cytometry. RESULTS: The subclone cell lines SK-BR-3/NDRG2-flag expressing NDRG2 stably and highly were successfully selected. The high expression of NDRG2 induced weak proliferation of breast cancer cell line( P < 0. 01 ) and the cells were arrested at G0/G1 phase( P < 0. 01). CONCLUSION: Over-expression of NDRG2 in SK-BR-3 cells effectively inhibits cell proliferation and induces cell cycle arrest.
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