Effect of vitamin D analogue EB1089 on proliferation and apoptosis of human laryngeal carcinoma cell line Hep-2
AIM: To explore the effect of EB1089 on the apoptosis of human laryngeal carcinoma Hep-2 cells and its mechanism. METHODS: Hep-2 cells were cultured and treated with EB1089 of different concentrations(0,1,10,100 nmol/L) for 24,48 and 96 h respectively. The growth inhibition rate of Hep-2 cells was detected by MTT method and the cell apoptosis rate was analyzed by TUNEL using fluorescence microscope. Caspase-3 activity was assessed by colorimetric assay. RESULTS: EB1089 inhibited the proliferation of Hep-2 cells in a time- and dose-dependent manner (P < 0. 05),with the inhibitory rate ranging from 2. 6% -70.0%. Forty-eight hours after EB1089 treatment at 10 nmol/L and 100 nmol/L,the apoptosis index of Hep-2 cells was 22. 8 ± 1.6 and 53.8±3.6,respectively. After 10 nmol/L EB1089 treatment for 12,24,48 and 96 h,the activities of caspase-3 were significantly increased respectively by 2. 09,2. 72,4. 91 and 5.23 folds compared with those in control group ( P < 0.05 ). The EB1089-induced apoptosis of Hep-2 cell was partly blocked with the treatment of caspase-3 inhibitor,Z-DEVD-FMK. CONCLUSION:EB1089 inhibits the proliferation and induces the apoptosis of Hep-2 cells by increasing caspase-3 activity.
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万方数据
维普
