氧化苦参碱和粉防己碱对鼻咽癌耐药细胞株的耐药逆转作用
Reversal effect of oxymatrine and tetrandrine on multidrug resistance in HNE-1(200) cell line
叶琳 1王驰 2陈鸿雁 3谢明均4
作者信息
- 1. 重庆医科大学,附属第一医院胸心外科,重庆,400016
- 2. 重庆医科大学,药学院,重庆,400016
- 3. 重庆医科大学,附属第一医院耳鼻喉科,重庆,400016
- 4. 宜宾市第二人民医院,四川,宜宾,644000
- 折叠
摘要
目的:研究天然有效成分氧化苦参碱(Oxy)和粉防己碱(TET)对鼻咽癌耐药细胞株HNE-1(200)的耐药逆转作用.方法:以鼻咽癌耐药细胞株HNE-1(200)为研究对象,用MTT法测定无毒剂量的Oxy和TET作用后HNE-1(200)耐药细胞对常用抗肿瘤药物顺铂(DDP),5-氟尿嘧啶(5-Fu),长春新碱(vincristine,VCR),阿霉素 (Adriamycin,ADM)的增殖抑制率和IC50,并进一步计算其逆转指数.结果:无毒剂量的TET和Oxy分别与4种抗肿瘤药物联合应用后,抗肿瘤药物对耐药细胞的增殖抑制率增加.TET逆转HNE-1(200)细胞对DDP,5-Fu,VCR,ADM的耐药指数分别为2.25,2.1,3.19,2.27;Oxy 逆转HNE-1(200)细胞对DDP,5-Fu,VCR,ADM的耐药指数分别为1.59,1.26,2.28,1.26.结论:低毒浓度的天然药物Oxy和TET对鼻咽耐药细胞株HNE-1(200)具有一定的耐约逆转作用.
Abstract
AIM:To study the reversal effects of natural substances oxymatrine and tetrandrine on multidrug resistance in human nasopharyngeal carcinoma multidrug resistance HNE-1 (200) cell line. METHODS: Human nasopharyngeal carcinoma multi-drug resistance HNE-1 (200) cell line was used as the subject. MTT reduction assay was used to investigate the drug interaction of oxymatrine,tetrandrine,cisplatin(DDP),5-Fluorouracil(5-Fu),vincristine(VCR) and adriamycin ( ADM) on HNE-1 (200) cell line. IC50 and RI were detected. RESULTS: After non-toxic dose of tetrandrine was interacted with DDP,5-Fu,VCR and ADM,the cell inhibition rate of the four drugs increased. The resistance index (RI) of tetandrine with DDP,5-Fu,VCR and ADM was 2. 25,2. 1,3. 19 and 2. 27 respectively. The RI of oxymatrine with DDP,5-Fu,VCR and ADM was 1. 59,1. 26,2.28 and 1. 26 respectively. CONCLUSION: Oxymatrine and tetrandrine can reverse the multidrug resistance of HNE-1 ( 200) cell line to DDP,5-Fu,VCR and ADM.
关键词
氧化苦参碱/粉防己碱/鼻咽肿瘤/HNE-1(200)细胞系/多药耐药Key words
oxymatrine/tetrandrine/nasopharyngeal neoplasms/HNE-1 (200) cell line/multidrug resistance引用本文复制引用
基金项目
重庆市卫生局中医处资助课题(渝中医[2003]32号2003-B-90)
出版年
2009
NETL
NSTL
维普
万方数据