白眉蝮蛇蛇毒细胞毒素H1对实验性大鼠胃癌抑制作用的实验研究
Inhibitory effect of cytotoxin from Agkitrodon Acutus venom on gastric cancer
鲁珏 1徐飞鹏1
作者信息
- 1. 广东医学院附属医院胃肠外科,广东,湛江,524000
- 折叠
摘要
目的:从白屑蝮蛇蛇毒中提取细胞毒素H1,观察细胞毒素H1对实验性大鼠胃癌的抑制作用.方法:采用DEAE-Sephadex A-50,TSKgel Pheny柱层析从白眉蝮蛇蛇毒中分离纯化细胞毒素H1.在饮水中添加致癌剂1-甲基-3-硝基-1-亚硝基胍(MNNG),增溶剂吐温20及维生素D3饲喂Wistar大鼠,并应用高张盐水间歇辅以刺激,制造大鼠胃癌模型,将存活的大鼠胃癌模型随机分为阴性对照组、5-Fu阳性对照组、细胞毒素H1给药组,连续注射3 wk.结果:细胞毒素H1给药组原位肿瘤体积、瘤质量较5-Fu组明显降低,具有统计学意义(P＜0.05).结论:细胞毒素H1确有抑制实验性大鼠胃癌生长和转移的作用.
Abstract
AIM: To purify cytotoxin from Agkitrodon Acutus venom and to study its inhibitory effect on gastric cancer in rats. METHODS: Cytotoxin H1 was isolated and purified by successive chromatography on DEAE-Sephadex A-50 and TSKgel Pheny columns from Agkitrodon acutus venom. To establish the gastric cancer model,the rats were fed with water added N-Methyl-N'-Nitro-Nitrosoguanidine,Tween 20 and Vitamin D3 and were stimulated with hypertonicsaline. The established models were randomly divided into negative control group,5-FU positive control group and treatment group of Cytotoxin H1 and were treated with continuous injection for 3 weeks. RESULTS: In situ tumor volume and tumor weight in treatment group was significantly lower than those in 5-FU group(P<0.05). CONCLUSION: Cytotoxic H1 inhibits gastric cancer growth and metastasis in rats.
关键词
细胞毒素/白眉蝮蛇毒/大鼠/胃肿瘤Key words
cytotoxin/agkitrodon acutus venom/rats/gastric stomach neoplasms引用本文复制引用
基金项目
湛江市科技攻关项目(2008C08006)
出版年
2009
NETL
NSTL
维普
万方数据