Regeneration of sciatic nerve allografts preserved in vitrification solution containing tetramethylpyrazine at -20℃
AIM: To study the regeneration of sciatic nerves allografts preserved in the vitrification solution containing tetramethylpyrazine at - 20℃ within different time period. METHODS: Sciatic nerves taken from male SD rats were preserved in vitrification solution containing 200 mg/L tetramethylpyrazine at - 20℃ for 1-2 weeks respectively ( Group A). The segments of these nerves were then transplanted to the corresponding Wistar rats with part of sciatic nerve defects. The allografts were examined with the gross observation,electrophysiology,immunofluorescence and counts of motor end-plate postoperatively. The results were compared respectively between Group A,Group B (vitrification solution at - 201) and Group C (vitrification solution containing tetramethylpyrazine at -196℃). RESULTS: No statistical difference was found between Group A and Group C,but significantly different was seen between Group A and Group B (P <0.01 ) for the regeneration of allografts preserved for 1 week. After the 2-week preservation,the regeneration was the best in Group C and the worst in Group B. CONCLUSION: The preserved temperature of peripheral nerve allografts can be raised by adding tetramethylpyrazine to the vitrification solution,in which peripheral nerve allografts can be preserved at - 20℃ for 1 week.
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万方数据
维普
