Effect of methoxychlor on implantation in mice exposed to HOXA-10 gene expression
ATM: To investigate the effects of methoxychlor (MXC) on HOXA-10 in mice in embryo implantation period. METHODS: By different dosage levels of MXC[0,16,32 and 64 mg/(kg · d) ],40 female Kunming mice aged 5 weeks were randomly divided into control group ( sesame oil),low-dosage group,mid-dosage group and high-dosage group,with 10 animals in each group. After 2 weeks of adaptive feeding,all the mice were given intraperitoneal injection of MXC for 15 d. Female and male mice were then put into one cage by the ratio of 2:1 ( female: male) for 4 d,during which intraperitoneal injection of MXC was continued. The mice were sacrificed on the 5th day of conception and the effect of MXC on uterus of mice was observed. RESULTS: After MXC injection,no abnormality was observed in the general state of the mice. Immunofluorescence assay showed that marked fluorescent light was observed in control group compared with that in MXC injection group. Real-time PCR showed that the expression of HOXA-10 mRNA in mid-dosage group and high-dosage group was significantly lower than that in control group ( P < 0. 05 ). CONCLUSION: MXC affects reproductive function by reducing the expression of HOXA-10 in uterus of mice.
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NSTL
万方数据
维普
