Protective effects of estradiol on angiopoietin-1 mRNA expression and bcl-2 gene expression in castrated rats with focal cerebral ischemia after reperfusion
AIM: To investigate the protective effect of estradiol on angiopoietin-1 mRNA ( Ang-1 mRNA) and bcl-2 gene expression in castrated rats with focal cerebral ischemia reperfusion. METHODS: Cerebral ischemia reperfusion models were established in female Wistar rats and the rats were divided into WT castrated rat placebo group ( A group),estradiol benzoate group ( B group),ERKO castrated rat placebo group(C group) and estradiol benzoate group(D group). The plasma estradiol levels,changes in cerebral blood flow,Ang-1 mRNA,apoptosis and bcl-2 gene expressions were determined respectively by radio-immunity analysis,laser Doppler flowmetry ( LDF),immunohistochemical stain and reverse transcription polymerase chain reaction ( RT-PCR). RESULTS: The plasma estradiol levels in WT and ERKO castrated rats treated with estradiol were higher than those in respective control groups( P <0.01). In group of WT MCAO castrated rats treated with estradiol,the change in cerebral blood flow after cerebral ischemia reperfusion,the Ang-1 mRNA expression and bcl-2 gene expression in brain tissues were higher than those for their respective control (P < 0. 01). CONCLUSION: The increased expression of Ang-1 mRNA and bcl-2 gene expression mediated by estradiol and their stabilizing effect on capillary vessels are the important repair mechanism of cerebral ischemia reperfusion injury.
NETL
NSTL
万方数据
维普
