摘要
目的:探讨α-硫辛酸(LA)对染铅大鼠骨显微结构及骨髓生化指标的影响.方法:将SD大鼠随机分为2组,空白对照组和铅染毒组(230 mg/L的醋酸铅灌胃),40 d后各组取3只大鼠测血铅和骨铅,将已染铅大鼠随机分为4组,即铅对照组、3个LA治疗组[30,60,100 mg/(kg·d)].LA治疗80d后处死动物,测定血铅、骨铅、HE染色观察骨小梁结构,免疫组化法检测碱性磷酸酶表达.结果:①LA各治疗组大鼠血铅、骨铅水平均低于铅对照组,差异有统计学意义(P＜0.05).②LA 100 mg治疗组大鼠,HE染色观察骨小梁结构,碱性磷酸酶表达与铅对照组比较,差异有统计学意义(P＜0.05).结论:LA能够拮抗铅的骨毒性.
Abstract
AIM: To determine the effects of α-lipoic acid on bone microstructure and bone marrow biochemical indicators in lead poisoned rats. METHODS: SD rats were divided into 2 groups: control group and lead group treated with lead acetate [ 230 ppm]. After 40 d,3 rats from each group were taken for the measurement of lead in blood and bone. The rats in lead group were then randomly subdivided into 4 groups: 1 lead control group and 3 lead groups with different concentrations of α-lipoic acid [30,60 and 100 mg/(kg · d) ]. At the end of the experiment (80 d),the rats were killed and the samples of blood and bone were taken for examination. RESULTS: Blood lead contents in every lead α-lipoic group were lower than those in lead control group(P<0.05). HE staining showed that after treatment with α-lipoic acid,the bone microstructure and AKP immunity class brood in 100 mg lead α-lipoic acid group were significantly different from those in lead control group (P < 0.05). CONCLUSION: α-lipoic acid antagonizes lead toxicity in bone marrow.
NETL
NSTL
维普
万方数据