Detection of chlamydia trachomatis and ureaplasma urealyticum using fluorescence polarization based on a multiple and asymmetric PCR
AIM: To develop a simple,economical,accurate and practical method for detection of chlamydia trachomatis and ureaplasma urealyticum simultaneously by fluorescence polarization ( FP) assay based on a multiple and asymmetric PCR. METHODS: Two sets of primers were used to amplify chlamydia trachomatis and ureaplasma urealyticum DNA by a multiple and asymmetric PCR. The probes of chlamydia trachomatis and ureaplasma urealyticum labeled with different fluorophores were hybridized respectively with target amplicons. The infection was determined by the increased FP value. Two hundred and forty-six samples were subjected to common assay to evaluate the feasibility of this method. RESULTS: Of the 246 samples,56 samples were identified as positive for chlamydia trachomatis,62 samples were identified as positive for ureaplasma urealyticum and 28 samples were identified as positive for double infection by the multiple PCR-FP assay. The results obtained by the multiple PCR-FP assay were all correct compared with the results by common assay. CONCLUSION: The proposed method allows a special,simple and economical detection for the simultaneous detection of chlamydia trachomatis and ureaplasma urealyticum by FP assay based on a multiple and asymmetric PCR.
NETL
NSTL
万方数据
维普
