Inhibitory effects of siRNA on expression of epidermal growth factor receptor in squamous cell carcinoma cell line A431
AIM: To investigate the inhibitory effect of RNA interference ( RNAi) on the epidermal growth factor receptor (EGFR) gene expression in A431 cell line. METHODS: Two pairs of small interfering RNA ( siRNA) for EGFR gene were designed and transfected into A431 cells. EGFR gene expression was assessed by RT-PCR and EGFR protein expression was tested by Western blot. The proliferation of the A431 cells was detected by MTT method and flow cytometry was used to measure the cell apoptosis. RESULTS: The expression rates of EGFR mRNA and the expression rates of EGFR protein were (16. 5 ± 1. 6 ) % and (11.1 ±1.6)%,respectively in A431 EGFR siRNA2-transfected cells,significantly lower than those of control cells (P < 0. 05). Obvious decrease in growth of the A431 cells was observed after transfected with EGFR siRNA2 and the apoptosis rate was (12.6 ±1.4)%,significantly higher than that of control cells (P < 0.05). CONCLUSION: siRNA effectively inhibits the transcription and the expression of EGFR gene in the squamous cell carcinoma cell line A431. siRNA increases apoptosis index of the A431 cells and inhibits their growth.
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