首页|苦参素对LPS诱导的人肾小球系膜细胞中p-STAT3和SOCS-3的影响

苦参素对LPS诱导的人肾小球系膜细胞中p-STAT3和SOCS-3的影响

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目的:观察苦参素对LPS诱导的人肾小球系膜细胞(HMC)中磷酸化信号转导和转录活化因子3(p-STAT3)和细胞因子信号抑制因子3(SOCS3)的表达及相互关系.方法:体外原代培养的HMC,分为正常组、LPS(10 mg/L)组、LPS(10mg/L)+苦参素(320 mg/L)组,每组分3个时间点(12,24,48h).采用MTT法检测HMC的增殖,RT-PCR方法检测STAT3和SOCS3 mRNA的表达,Western Blot方法检测p-STAT3和SOCS3蛋白的表达.结果:苦参素能明显抑制LPS诱导的HMC增殖,与正常组相比,p-STAT3和STAT3 mRNA的表达在12,24,48 h均上调,SOCS3蛋白和mRNA的表达也同步上调.与LPS诱导组相比,苦参素组p-STAT3和STAT3 mRNA的表达在12,24,48 h均下调,而SOCS3蛋白和mRNA表达有明显上调的趋势.结论:苦参素能抑制LPS诱导的HMC的增殖,且能上调LPS诱导的HMC中SOCS3 mRNA和蛋白表达,下调P-STAT3和STAT3 mRNA的表达,其作用可能与上调SOCS3、抑制STAT3磷酸化有关.
Effect of Oxymatrine on expression of p-STAT3 and SOCS3 in lipopolysaccharide-induced human glomerularmesangial cells
AIM:To observe the effects of Oxymatrine on the phosphorylation expression of signal transducers and activators of transcription 3 (p-STAT3 ) and suppressors of cytokine signaling 3 (S0CS3) in lipopolysaccharide (LPS)-induced human glomeru-larmesangial cells (HMCs). METHODS: HMCs cultured in vitro were divided into 3 groups: control group,LPS (10 mg/L) group and OM group ( LPS 10 mg/L + Oxymatrine 320 mg/L). HMCs proliferation was detected by MTT assay,protein expressions of p-STAT3 and SOCS3 were observed by Western blot,and STAT3 and S0CS3 mRNA were measured by real-time quantitative RT-PCR. RESULTS: Compared with those in normal control group,the expressions of p-STAT3,STAT3 mRNA,SOCS3 and SOCS3 mRNA all significantly increased in PS-induced HMCs. Compared with those in LPS group,the expressions of p-STAT3 and STAT3 mRNA decreased in OM group,but the expression changes of SOCS3 and SOCS3 mRNA displayed an increasing tendency. CONCLUSION: Oxymarine inhibits the proliferation of PS-induced HMCs and increases the expressions of SOCS3 and SOCS3 mRNA. Oxymarine also decreases the expressions of P-STAT3 and STAT3 mRNA,possibly by increasing SOCS3 to inhibit the expressions of p-STAT3.

glomerular mesangium/cytologycell proliferationphosphorylation of signal transducers and activators of transcription 3suppressors of cytokine signaling 3oxymatrinelipopolysaccharides

凌继祖、金玉、李宇宁、党红星、苏婕

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兰州大学基础医学院,甘肃,兰州,730000

兰州大学第一医院儿科,甘肃,兰州,730000

兰州大学第一临床医学院,甘肃,兰州,730000

肾小球膜/细胞学 细胞增殖 磷酸化细胞信号转导及转录活化因子3 细胞因子信号抑制因子3 氧化苦参碱 脂多糖类

甘肃省中医药科研项目兰州大学医学科研基金

GZK-2008-5LZUYX200631

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子：0.599
ISSN：1000-2790
年,卷(期)：2009.30(12)
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