Signal pathway of calcineurin-inhibitor in cardiac hypertrophy of renovascular hypertensive rats
AIM:To investigate the signal pathway of myocardial calcineurin inhibitor (Cain) in renohypertensive rats treated with 1-kidney-1-clamp (1k1c) operation and to study the changes of related active factors in plasma. METHODS: Twenty-one male Wistar rats were randomly divided into 3 groups ( n = 7) : sham operatinggroup,operating group treated with 1k1c operation and cyclosporin A ( CgA) group treated with CsA 5 mg/( kg· d) after the 1k1c operation. Fourteen days after the treatment,AngⅡ and Ald levels in plasma were determined by radioimmunoassay and the expression of Cain in myocardium was determined by Western Blot. RESULTS: The Ald and AngⅡ levels,the expression of Cain and the activity of calcineurin in myocardium increased in operating group compared with those in sham operating group while the Ald and Ang Ⅱ levels,the expression of myocardial Cain and the activity of calcineurin significantly decreased in CsA group compared with those in operating group. CONCLUSION: The 1k1c operation induces cardiac hypertrophy through a calci-neurin-depedent signal pathway. The expression of internal Cain may be controlled by the activity of calcineurin.
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万方数据
维普
