siRNA干扰cerbB1对人脑膜瘤细胞基因表达及增殖抑制的影响
Effect of specific siRNA targeting cerbB1 on EGFR and survivin expression and cell proliferation in human meningioams
刘重霄 1师蔚 1高李贵 1孙建军 1周乐 1王睿智 1郭振宇 1卢兴苗1
作者信息
- 1. 西安交通大学医学院第二附属医院神经外科,陕西,西安,710004
- 折叠
摘要
目的:探讨靶向cerbB1的siRNA对脑膜瘤细胞增殖以及EGFR和survivin表达的影响.方法:设计并合成针对cerbB1的siRNA-1,将其转染至培养的人脑膜瘤细胞,利用流式细胞仪和MTY法检测其对脑膜瘤细胞增殖和凋亡的影响,并采用Wesmm Blot和RT-PCR法检测干扰后脑膜瘤细胞EG-FR和survivin mRNA和蛋白表达变化.结果:靶向cerbB1的siRNA-1显著抑制了培养脑膜瘤细胞增殖(P<0.05),增加了siRNA-1组早期、晚期凋亡细胞和坏死细胞(P<0.01). West-ern Blot和RT-PCR法结果显示,siRNA-1组脑膜瘤细胞EGFR和survivin mRNA和蛋白表达明显降低(P<0.01).结论:cerbB1特异性siRNA可明显下调脑膜瘤细胞cerbB1和sur-vivin基因表达,并能有效抑制脑膜瘤细胞增殖,诱导其凋亡,为脑膜瘤的靶向治疗提供实验基础.
Abstract
AIM: To study the influence of siRNA targeting cerbBl on cell proliferation and expression of EGFR and survivin in cultured human meningioma cells. METHODS: Small interfering RNA ( siRNA) targeting cerbB1 was constructed and transfected into human meningioina cells in vitro. Flow cytometry and MTT method were used to detect the effect of siRNA on the proliferation and apoptosis of cultured human meningioma cells. The expression of EGFR and survivin was analyzed by Western Blot and RT-PCR after siRNA interference in meningioma cells. RESULTS: The meningioma cell proliferation was inhibited significantly after siRNA interference,the apoptosis and necrosis cells in siRNA group increased significantly,and the expression of EGFR and survivin was significantly down-regulated after interference. CONCLUSION: siRNA targeting cerbBl significantly down-regulates the expression of EGFR and survivin,inhibits the proliferation and induces the apoptosis of meningioma cells. The findings of this study provide some experimental base for the gene targeting treatment of meningiomas.
关键词
脑膜瘤/RNA,小分子干扰/细胞凋亡/受体,表皮生长因子/survivinKey words
meningioma/RNA,small interfering/apoptosis/receptor,epidermal growth factor/survivin引用本文复制引用
基金项目
陕西省科技计划项目(2004K13-G17)
西安交通大学第二医院科研基金(2003-YL-14)
出版年
2009
NETL
NSTL
维普
万方数据