目的:建立荷人膀胱癌的原位动物模型,通过磁共振成像(MRI)监测肿瘤生长过程,并利用此模型评价华蟾素抗膀胱癌作用.方法:直视下经尿道机械BALB/c裸鼠膀胱黏膜,将人膀胱癌细胞T24经尿道种植于25只裸鼠膀胱,建立荷人膀胱癌原位动物模型.以钆-二乙三胺五乙酸作为膀胱造影剂,用MRI监测肿瘤的生长,同时取裸鼠膀胱组织标本行HE染色进行病理学检奁.同法建立膀胱癌动物模型18只,分为对照组和给药组,各9只.膀胱灌注6次后,荷瘤膀胱称质量.结果:25只裸鼠在种植T24细胞后均形成膀胱肿瘤.种植后7 d,裸鼠膀胱MRI检查无明显变化,14,21,28 d MRI检查均可发现膀胱不同程度的充盈缺损,MRI图像与肿瘤实际大小吻合.病理检查显示,种植后7 d,肿瘤生长于裸鼠膀胱黏膜或浅层肌肉,14～28 d局限于肌层,35 d时侵及浆膜层.华蟾素给药组抑瘤率为46.9%.结论:成功建立了裸鼠原位膀胱癌动物模型,肿瘤生长基本模拟了人膀胱癌的发生、发展过程,MRI检查可作为对裸鼠膀胱原位肿瘤动态观察的可靠方法,华蟾素确有明显的抗膀胱癌的作用.
Effect of cinobufacin on ort orthotopic murine bladder cancer
AIM: To establish orthotopic animal models bearing the human bladder cancer and evaluate the anti-tumor effort of cinobufacin on bladder cancers. METHODS: Human bladder cancer cell line T24 was inoculated into the bladders of 25 BALB/c nude mice to establish orthotopic bladder cancer models. Eighteen mice bearing bladder cancers were randomized into 2 groups: untreated group and experiment group. Magnetic resonance imaging (MRI),using Gd-DTPA as contrast agent,was performed to monitor the tumor progression. The pathologic morphology of the bladders was observed. The bladders were weighed after 6 intra-vesical infusions. RESULTS: All the 25 mice developed bladder cancers after T24 cell inoculation. Seven days after inoculation,no changes were observed in the bladders by MRI and filling defects in the bladders,accordant to the actual tumor size,were seen by MRI at 14,21 and 28 d after inoculation. Pathologic examination showed that tumors grew in mucosa of the bladders at 7 d after inoculation,infiltrated into muscle layer at 14 -28 d after inoculation,and invaded serosa at 35 d after inoculation. The inhibition rate of the tumor growth in the experiment group was 46.9%. CONCLUSION: We have successfully established the orthotopic bladder cancer model,which mostly simulates the progression of the human bladder cancer. MRI is a reliable way for dynamic detections of murine orthotopic bladder tumors. Cinobu-fotalin has some obvious anti - rumor effect on bladder cancers.
NETL
NSTL
万方数据
维普
