Extraction of anti-neoplasm active component from Gekko swinhonis and its inhibitory effects on CT-26 murine colon carcinoma
AIM:To study anti-neoplasm activity of the pharma-codynamic component of Gekko swinhoni. METHODS:The phar-macodynamic component of Gekko swinhoni was extracted. Anti-neoplasm activity of Gekko swinhoni pharmacodynamic component was evaluated by MTT assay in order to measure the inhibition rate of tumor cell growth. CT-26 murine colon carcinoma model of BALB/c male mice were randomly divided into groups. According to the weights,the Gekko swinhonis anti-neoplasm active groups were divided into dose of 0.25,0.10 and 0.05 mg/kg respectively,the positive control group of ES was administrated to 6 mg/kg,the negative control group was treated with an equal volume of saline,then axillary injection once a day for every group. After 15 d,the experimental mice were putted to death by dislocation to measure body weight,then dissect subcutaneouly tumor and measure humid weight. RESULTS: The extract of Gekko swinhonis has showed excellent anti-neoplasm activity and can inhibit cell proliferate in a time- and dose-dependent manner. The highest tumor inhibitory rate is up to 45.50% in vitro experiments and is up to 67.24% in vivo experiments. CONCLUSION: Gekko swinhonis is deserved to develop and study for its splendiferous anti-neoplasm activity on CT-26.
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维普
