Identification of serum tumormarkers for diagnosing stage Ⅰ lung squamous cell carcinoma by MALDI-TOF mass spectrometry
AIM: To identify specific tumor markers for more effective early diagnosis of lung squamous cell carcinoma using matrix-assisted laser desorption utilization rime of flight mass spectrometry (MALDI-TOF-MS) technology. METHODS: Serum samples were isolated from 19 patients with stage I lung squamous cell carcinoma and 19 age- and smoking-matched healthy controls,and the serum proteomic profiles were obtained by MALDI-TOF-MS. RESULTS: Compared with those in healthy control group,three highly-expressed potential tumor markers were identified with the respective molecular weights of 3261.69,3192.07 and 2556.92(P<0. 01). The three best novel protein peaks were automatically chosen for the system training and the development of the constructed model. The constructed model was then used to test an independent set of masked serum samples from 22 patients with lung squamous cell carcinoma and 19 healthy individuals. The analysis yielded a sensitivity of 95. 5% and a specificity of 94.7%. CONCLUSION: These results suggest that MALDI-TOF-MS protein chip technology is a quick,convenient and high-output analyzing method. This method is capable of selecting several relatively potential tumor markers from the serum of patients with lung squamous cell carcinoma,is of good values in clinical application and will provide clues to new serologic tumor markers of lung squamous cell carcinoma.
NETL
NSTL
万方数据
维普
