首页|环氧化酶2启动子区基因多态性与慢性牙周炎的关系

环氧化酶2启动子区基因多态性与慢性牙周炎的关系

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目的:研究环氧化酶2启动子区-765位点、-1195位点单核苷酸多态性与慢性牙周炎遗传易感性的关系.方法:根据慢性牙周炎的诊断及纳入标准收集150例慢性牙周炎患者及143名健康对照者,检查牙周状况并根据牙周附着丧失的程度进行分类,用问卷调查了解受试者的一般情况.取患者的颊黏膜拭子提取其基因组DNA,采用多聚酶链反应-限制性内切酶片段长度多态性的方法检测环氧化酶2-765位点G>C及-1195位点G>A的基因型分布并分析其基因多态性与慢性牙周炎易感性的关系.结果:环氧化酶2-765位点G>C基因型频率在各组间无显著性差异,但-1195位点G>A的基因型分布在中、重度牙周炎患者较健康对照者之间有统计学差异(P<0.05),携带GA或从基因型可使牙周病的患病风险增加(OR3.3,95%CI 1.6～6.9或OR3.7,95%CI 1.5～9.5),中、重度牙周炎患者从或GA基因型频率较健康对照者显著增加.结论:环氧化酶2启动子区位点单核苷酸多态性与慢性牙周炎的易感性有关.
Association of cyclooxygenase-2 gene polymorphisms in promoter region with chronic periodontitis
AIM: To evaluate the association of cyclooxygenase-2( cox-2) single-nucleotide polymorphisms ( SNP) in the promoter region ( -1195G>A,-765G>C) and susceptibility to chronic periodontitis. METHODS: One hundred and fifty cases of chronic periodontitis (CP) and 143 cases of healthy controls (HC) were recruited for the study. Genotypes of cox-2 ( - 765G > C, -1195G > A) were determined by polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) method with genomic DNA extracted from buccal mucosa swab. The distribution of genotypes between the groups was compared and the risks for periodontitis associated with genotypes were assessed. RESULTS: The -765G > C SNP was not significantly associated with the risk of chronic periodontitis. However,the risk of CP was significantly higher in the presence of genotype GA and AA for -1195G>A (OR3.3,95% CI 1.6-6.9 and OR3.7,95% CI 1. 5 - 9. 5). The severity of CP was associated with cox-2 -1195G > A polymorphisms. CONCLUSION: Our study reveals some possible influence of single-nucleotide polymorphisms for - 1195G > A in chronic periodontitis.

prostaglandin-endoperoxide synthese-2chronic diseaseperiodontitisgenetic predisposition to diseasepolymorphism restriction fragment lengthpolymerase chain reaction

孙书昱、曾雄群、靳安民、范卫华、章锦才

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南方医科大学附属口腔医院牙周科,广东,广州,510280

南方医科大学附属珠江医院,广东,广州,510280

前列腺素内过氧化物合酶2 慢性病 牙周炎 疾病遗传易感性 多态性,限制性片段长度 聚合酶链式反应

广东省自然科学基金国家科技攻关项目

060229422007BAI18B02

2009

第四军医大学学报
第四军医大学

第四军医大学学报

CSTPCDCSCD北大核心
影响因子：0.599
ISSN：1000-2790
年,卷(期)：2009.30(12)
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