Expression of zinc finger protein A20 of mouse lung tissues after acute spinal cord injury
AIM: To study the expression of endogenous zinc finger protein A20 of mouse lung tissues in the process of spinal cord injury (SCI) and/or hpopolysaccharide (LPS) infection. METHODS : A total of 220 healthy mice ( from Kunming, Yun-nan Province ) were randomized into 4 groups: control group (group N), SCI group(group A), LPS group(group B) and SCI plus LPS group(group AB). The expressions of A20 protein and mRNA were measured by immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction(RT-PCR) and the degree of pathological lesion was detected by HE staining. RESULTS: Both A20 protein and A20 mRNA expressed at low levels in group N and the highest expressions of A20 protein and A20 mRNA were observed in group AB, which reached peak respectively at 8 h and at 4 h, In group AB, severe pathologic damage of lung tissues was observed at 0.5 h. The severest damage was seen at 4 h and it began to alleviate after 8 h. CONCLUSION: In the early period of SCI, the expression of zinc finger protein A20 is high, especially in the case of LPS infection. The increased expression of A20 following SCI(or with LPS infection) may play an important role in regulating inflamma-tory response.
NETL
NSTL
万方数据
维普
